Trial by fire: are the crystals macromolecules?

نویسندگان

  • Kannan Raghunathan
  • Paul T Harris
  • Dennis N Arvidson
چکیده

Protein crystallization screens frequently yield salt crystals as well as protein crystals. A simple method for determining whether a crystal is composed of salt or macromolecules is suggested. A drop containing one or more crystals is transferred to a glass cover slip and the cover slip is then passed through the flame of a Bunsen burner. Macromolecule crystals are destroyed by this treatment, while salt crystals generally remain. The test can be performed after other commonly used tests such as crushing and staining.

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عنوان ژورنال:
  • Acta crystallographica. Section F, Structural biology and crystallization communications

دوره 66 Pt 5  شماره 

صفحات  -

تاریخ انتشار 2010